Photomodulation alleviates cellular senescence of aging adipose-derived stem cells.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) therapies are emerging as a promising approach to therapeutic regeneration. Therapeutic persistence and reduced functional stem cells following cell delivery remain critical hurdles for clinical investigation due to the senescence of freshly isolated cells and extensive in-vitro passage.

Methods

Cultured adipose-derived stem cells (ASCs) were derived from subcutaneous white adipose tissue isolated from mice fed a normal diet. We performed senescence-associated-β-galactosidase (SA-β-gal) staining, real-time PCR, and Westernblot to evaluate the levels related to cellular senescence markers.

Results

The mRNA expression levels of senescence markers were significantly increased in the later passage of ASCs. We show that light activa

SUBMITTER: Zhang T 

PROVIDER: S-EPMC10278352 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature