CircFAM114A2 inhibits the progression of hepatocellular carcinoma via miR-630/HHIP axis.

ABSTRACT:

Background

Many studies have shown that circular RNAs (circRNAs) are abnormally expressed in various tumor tissues and served as a key regulator in the occurrence and development of cancer. However, in hepatocellular carcinoma (HCC), the molecular mechanism of circRNAs in body fluids remains to be further explored.

Methods

The expression levels of genes and proteins were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting, respectively. Cell counting Kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU), wound healing assay, Transwell assays, flow cytometry, and tumor formation models in nude mice were conducted to investigate the effects of circFAM114A2 on HCC cells both in vitro and in vivo. RNA antisense purification (RAP), dual luciferase

SUBMITTER: Lai M 

PROVIDER: S-EPMC10278467 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature