Modified CTAB protocols for high‐molecular‐weight DNA extractions from ferns

ABSTRACT: Abstract

Premise

Efficient protocols for extracting high‐molecular‐weight (HMW) DNA from ferns facilitate the long‐read sequencing of their large and complex genomes. Here, we perform two cetyltrimethylammonium bromide (CTAB)‐based protocols to extract HMW DNA and evaluate their applicability in diverse fern taxa for the first time.

Methods and Results

We describe two modified CTAB protocols, with key adjustments to minimize mechanical disruption during lysis to prevent DNA shearing. One of these protocols uses a small amount of fresh tissue but yields a considerable quantity of HMW DNA with high efficiency. The other accommodates a large amount of input tissue, adopts an initial step of nuclei isolation, and thus ensures a high yield in a short period of time. Both metho

SUBMITTER: Xie P 

PROVIDER: S-EPMC10278929 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature