A comparison of freezer-stored DNA and herbarium tissue samples for chloroplast assembly and genome skimming.

ABSTRACT:

Premise

The use of DNA from herbarium specimens is an increasingly important source for evolutionary studies in plant biology, particularly in cases where species are rare or difficult to obtain. Here we compare the utility of DNA from herbarium tissues to their freezer-stored DNA counterparts via the Hawaiian Plant DNA Library.

Methods

Plants collected for the Hawaiian Plant DNA Library were simultaneously accessioned as herbarium specimens at the time of collection, from 1994-2019. Paired samples were sequenced using short-read sequencing and assessed for chloroplast assembly and nuclear gene recovery.

Results

Herbarium specimen-derived DNA was statistically more fragmented than freezer-stored DNA derived from fresh tissue, leading to poorer chloroplast assembly and

SUBMITTER: McAssey EV 

PROVIDER: S-EPMC10278930 | biostudies-literature | 2023 May-Jun

REPOSITORIES: biostudies-literature