An optimized RNA extraction method for diverse leaves of Hawaiian Metrosideros, a hypervariable tree species complex

ABSTRACT: Abstract

Premise

The isolation of RNA from trees is challenging due to the interference of polyphenols and polysaccharides with downstream processes. Furthermore, many RNA extraction protocols are time consuming and involve hazardous chemicals. To address these issues, we aimed to develop a safe protocol for high‐quality RNA extraction from diverse Metrosideros taxa representing a broad range of leaf toughness, pubescence, and secondary metabolites.

Methods and Results

We tested popular RNA isolation kits and protocols that were effective on other recalcitrant trees, including a broad range of optimization and purification steps. We optimized a protocol involving two silica‐membrane column‐based kits that yielded high‐quantity RNA with an RNA integrity number >7 and witho

SUBMITTER: Hadi M 

PROVIDER: S-EPMC10278935 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature