Ethanol preservation and pretreatments facilitate quality DNA extractions in recalcitrant plant species.

ABSTRACT:

Premise

The preservation of plant tissues in ethanol is conventionally viewed as problematic. Here, we show that leaf preservation in ethanol combined with proteinase digestion can provide high-quality DNA extracts. Additionally, as a pretreatment, ethanol can facilitate DNA extraction for recalcitrant samples.

Methods

DNA was isolated from leaves preserved with 96% ethanol or from silica-desiccated leaf samples and herbarium fragments that were pretreated with ethanol. DNA was extracted from herbarium tissues using a special ethanol pretreatment protocol, and these extracts were compared with those obtained using the standard cetyltrimethylammonium bromide (CTAB) method.

Results

DNA extracted from tissue preserved in, or pretreated with, ethanol was less fragmented t

SUBMITTER: Johnson G 

PROVIDER: S-EPMC10278939 | biostudies-literature | 2023 May-Jun

REPOSITORIES: biostudies-literature