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PBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination.

ABSTRACT: Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype.

SUBMITTER: Boukthir S

PROVIDER: S-EPMC10279790 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination.

Boukthir S S Gaudu P P Faili A A Kayal S S

Heliyon 20230529 6

Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype. ...[more]

PMID: 37346331

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