Monitoring Caenorhabditis elegans molting in a conventional luminometer.

ABSTRACT: Molting is an essential developmental process in Caenorhabditis elegans. However, the study of molting in the worm has been limited by the lack of automated techniques that allow monitoring the process in a simple way. In 2015, Olmedo et al. published an automated method to monitor the timing of each larval stage and molt in C. elegans using bioluminescence. This new method has greatly contributed to the study of molting in this organism but requires the use of a high-sensitivity luminometer, which many laboratories do not have. We have adapted the method to a conventional luminometer, so that it can be used by most laboratories that work with C. elegans and do not have high-sensitivity equipment.•A customization of a method to study molting in C. elegans

SUBMITTER: Guijarro-Hernandez A 

PROVIDER: S-EPMC10279910 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature