Identification of novel coenzyme Q₁₀ biosynthetic proteins Coq11 and Coq12 in Schizosaccharomyces pombe.

ABSTRACT: Coenzyme Q (CoQ) is an essential component of the electron transport system in aerobic organisms. CoQ₁₀ has ten isoprene units in its quinone structure and is especially valuable as a food supplement. However, the CoQ biosynthetic pathway has not been fully elucidated, including synthesis of the p-hydroxybenzoic acid (PHB) precursor to form a quinone backbone. To identify the novel components of CoQ₁₀ synthesis, we investigated CoQ₁₀ production in 400 Schizosaccharomyces pombe gene-deleted strains in which individual mitochondrial proteins were lost. We found that deletion of coq11 (an S. cerevisiae COQ11 homolog) and a novel gene designated coq12 lowered CoQ levels to ∼4% of that of the WT strain. Addition of PHB or p-hydroxybenzaldehyde restored the CoQ c

SUBMITTER: Nishida I 

PROVIDER: S-EPMC10279924 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature