Establishment and characterization of adult human gastric epithelial progenitor-like cell lines.

ABSTRACT:

Objective

Human gastric epithelial stem/progenitor cells are important for stomach homeostasis; however, the in vitro culture system of these cells remains immature. Although three-dimensional (3D) organoid culture has fundamentally changed the in vitro study of gastrointestinal tract, its use is limited by inaccessible luminal compartment, and difficulties of imaging and manipulation. To overcome these limitations of 3D organoid culture system, we established adult human gastric epithelial progenitor-like (hGEPL) cell lines using a novel robust monolayer cell culture system.

Materials and methods

We established an in vitro gel-based monolayer culture system for normal human adult gastric epithelium, and compared it with traditional two-dimensional (2D) and 3D organoid cultu

SUBMITTER: Gao Y 

PROVIDER: S-EPMC10280148 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature