Large-scale antibody immune response mapping of splenic B cells and bone marrow plasma cells in a transgenic mouse model.

ABSTRACT: Molecular characterization of antibody immunity and human antibody discovery is mainly carried out using peripheral memory B cells, and occasionally plasmablasts, that express B cell receptors (BCRs) on their cell surface. Despite the importance of plasma cells (PCs) as the dominant source of circulating antibodies in serum, PCs are rarely utilized because they do not express surface BCRs and cannot be analyzed using antigen-based fluorescence-activated cell sorting. Here, we studied the antibodies encoded by the entire mature B cell populations, including PCs, and compared the antibody repertoires of bone marrow and spleen compartments elicited by immunization in a human immunoglobulin transgenic mouse strain. To circumvent prior technical limitations for analysis of plasma cells, we appl

SUBMITTER: Pan X 

PROVIDER: S-EPMC10280637 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature