NIPSNAP1 directs dual mechanisms to restrain senescence in cancer cells.

ABSTRACT:

Background

Although the executive pathways of senescence are known, the underlying control mechanisms are diverse and not fully understood, particularly how cancer cells avoid triggering senescence despite experiencing exacerbated stress conditions within the tumor microenvironment.

Methods

Mass spectrometry (MS)-based proteomic screening was used to identify differentially regulated genes in serum-starved hepatocellular carcinoma cells and RNAi employed to determine knockdown phenotypes of prioritized genes. Thereafter, gene function was investigated using cell proliferation assays (colony-formation, CCK-8, Edu incorporation and cell cycle) together with cellular senescence assays (SA-β-gal, SAHF and SASP). Gene overexpression and knockdown techniques were applied to examin

SUBMITTER: Gao E 

PROVIDER: S-EPMC10280965 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature