A platform for efficient establishment and drug-response profiling of high-grade serous ovarian cancer organoids.

ABSTRACT: The broad research use of organoids from high-grade serous ovarian cancer (HGSC) has been hampered by low culture success rates and limited availability of fresh tumor material. Here, we describe a method for generation and long-term expansion of HGSC organoids with efficacy markedly improved over previous reports (53% vs. 23%-38%). We established organoids from cryopreserved material, demonstrating the feasibility of using viably biobanked tissue for HGSC organoid derivation. Genomic, histologic, and single-cell transcriptomic analyses revealed that organoids recapitulated genetic and phenotypic features of original tumors. Organoid drug responses correlated with clinical treatment outcomes, although in a culture conditions-dependent manner and only in organoids maintained in human plasma

SUBMITTER: Senkowski W 

PROVIDER: S-EPMC10281085 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature