Copper-mediated oxidation of imidazopyrazinones inhibits marine luciferase activity.

ABSTRACT: The development of bioluminescence-based tools has seen steady growth in the field of chemical biology over the past few decades ranging in uses from reporter genes to assay development and targeted imaging. More recently, coelenterazine-utilizing luciferases such as Gaussia, Renilla, and the engineered nano-luciferases have been utilized due to their intense luminescence relative to firefly luciferin/luciferase. The emerging importance of these systems warrants investigations into the components that affect their light production. Previous work has reported that one marine luciferase, Gaussia, is potently inhibited by copper salt. The mechanism for inhibition was not elucidated but was hypothesized to occur via binding to the enzyme. In this study, we provide the first report of a group o

SUBMITTER: O'Sullivan JJ 

PROVIDER: S-EPMC10281456 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature