Normal cell cycle progression requires negative regulation of E2F1 by Groucho during S phase and its relief at G2 phase.

ABSTRACT: The cell cycle depends on a sequence of steps that are triggered and terminated via the synthesis and degradation of phase-specific transcripts and proteins. Although much is known about how stage-specific transcription is activated, less is understood about how inappropriate gene expression is suppressed. Here, we demonstrate that Groucho, the Drosophila orthologue of TLE1 and other related human transcriptional corepressors, regulates normal cell cycle progression in vivo. We show that, although Groucho is expressed throughout the cell cycle, its activity is selectively inactivated by phosphorylation, except in S phase when it negatively regulates E2F1. Constitutive Groucho activity, as well as its depletion and the consequent derepression of e2f1, cause cell cycle phenotypes. Our result

SUBMITTER: Bar-Cohen S 

PROVIDER: S-EPMC10281550 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature