Microsurgical Methods to Isolate and Culture the Early Gastrula Dorsal Marginal Zone.

ABSTRACT: Marginal zone explants from Xenopus embryos can be used to expose cell behaviors and tissue movements that normally operate in dorsal tissues. Dorsal explants comprise the diverse set of progenitor cells found in dorsal tissues including mesendoderm, head mesoderm, prechordal mesoderm, endoderm with bottle cells, axial mesoderm of the prospective notochord, paraxial mesoderm of the somites, lateral plate mesoderm, neural ectoderm, and ectoderm. Unlike an organoid, the dorsal marginal zone (DMZ) explant is "organotypic" in that microsurgery does not disrupt native tissue organization beyond manipulations needed to dissect the tissue from the embryo. An organotypic early gastrula DMZ explant preserves boundaries and close tissue associations in the native marginal zone. Depending on t

SUBMITTER: Davidson LA 

PROVIDER: S-EPMC10281795 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature