Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome.

ABSTRACT: The intestines of animals are colonized by commensal microbes, which impact host development, health, and behavior. Precise quantification of colonization is essential for studying the complex interactions between host and microbe both to validate the microbial composition and study its effects. Drosophila melanogaster, which has a low native microbial diversity and is economical to rear with defined microbial composition, has emerged as a model organism for studying the gut microbiome. Analyzing the microbiome of an individual organism requires identification of which microbial species are present and quantification of their absolute abundance. This article presents a method for the analysis of a large number of individual fly microbiomes. The flies are prepared in 96-well plates, enablin

SUBMITTER: Dodge R 

PROVIDER: S-EPMC10281796 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature