Modelling ligand depletion for simultaneous affinity and binding site quantification on cells and tissue.

ABSTRACT: The quantification of the number of targets in biological systems is an important parameter to assess the suitability of surface markers as targets for drugs, drug delivery and medical imaging. Likewise, quantifying the interaction with the target in terms of affinity and binding kinetics is essential during drug development. Commonly used approaches to quantify membrane antigens on live cells are based on manual saturation techniques that are labour-intensive, require careful calibration of the generated signal and do not quantify the binding rates. Here, we present how measuring interactions in real-time on live cells and tissue under ligand depletion conditions can be used to simultaneously quantify the kinetic binding parameters as well as the number of available binding sites in a bio

SUBMITTER: Weber J 

PROVIDER: S-EPMC10282064 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature