GDSC SMLM: Single-molecule localisation microscopy software for ImageJ.

ABSTRACT: Single-molecule localisation microscopy (SMLM) uses software to extract super-resolved positions from microscope images of fluorescent molecules. These localisations can then be used to render super-resolution images or analysed to extract information about molecular behaviour. The GDSC SMLM software provides a set of tools for analysing SMLM data in a single cross-platform environment. The software identifies fluorescent molecules in raw microscope images and localises their positions using stages of spot detection, spot fitting and spot rejection. The resulting localisation data set can then be visualised, cropped and filtered. A suite of downstream analysis tools enable the user to perform single-particle tracking, cluster analysis and drift correction. In addition, GDSC SMLM also provi

SUBMITTER: Etheridge TJ 

PROVIDER: S-EPMC10282564 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature