Dataset Information

Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater.

ABSTRACT:

Background

Salmonella enterica is one of the most prevalent bacterial foodborne pathogens. Salmonella phages are currently used in biocontrol applications and have potential for use as therapeutics.

Materials and methods

Phages were enriched and purified from a diversity of Salmonella host isolates. Morphology was determined with transmission electron microscopy, host ranges were characterized using an efficiency of plaquing assay, and comparative genomic analysis was performed to determine taxonomy.

Results

Ten phages were isolated and characterized. Phages showed activity against 23 out of the 24 Salmonella serovars evaluated. Two phages also showed activity against Escherichia coli strain B. Phages belonged to five different genera (Ithacavirus, Gelderlandvirus, Kuttervirus, Tlsvirus, and Epseptimavirus), two established species, and eight novel species.

Conclusions

The phages described here further demonstrate the diversity of S. enterica phages present in wastewater effluent. This work contributes a collection of characterized phages from eastern Tennessee that may be of use in future phage-based applications targeting S. enterica.

SUBMITTER: Bryan DW

PROVIDER: S-EPMC10282790 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Publications

Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater.

Bryan Daniel W DW Hudson Lauren K LK Wang Jia J Denes Thomas G TG

PHAGE (New Rochelle, N.Y.) 20230619 2

<h4>Background</h4><i>Salmonella enterica</i> is one of the most prevalent bacterial foodborne pathogens. <i>Salmonella</i> phages are currently used in biocontrol applications and have potential for use as therapeutics.<h4>Materials and methods</h4>Phages were enriched and purified from a diversity of <i>Salmonella</i> host isolates. Morphology was determined with transmission electron microscopy, host ranges were characterized using an efficiency of plaquing assay, and comparative genomic anal ...[more]

PMID: 37350991

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Project description:Bacteriophages (phages) outnumber bacteria ten-to-one and cause infections at a rate of 1025 per second. The ability of phages to reduce bacterial populations makes them attractive alternative antibacterials for use in combating the rise in antimicrobial resistance. This effort may be hindered due to bacterial defenses such as Bacteriophage Exclusion (BREX) that have arisen from the constant evolutionary battle between bacteria and phages. For phages to be widely accepted as therapeutics in Western medicine, more must be understood about bacteria-phage interactions and the outcomes of bacterial phage defense. Here, we present the annotated genomes of 12 novel bacteriophage species isolated from water sources in Durham, UK, during undergraduate practical classes. The collection includes diverse species from across known phylogenetic groups. Comparative analyses of two novel phages from the collection suggest they may be founding members of a new genus. Using this Durham phage collection, we determined that particular BREX defense systems were likely to confer a varied degree of resistance against an invading phage. We concluded that the number of BREX target motifs encoded in the phage genome was not proportional to the degree of susceptibility. IMPORTANCE Bacteriophages have long been the source of tools for biotechnology that are in everyday use in molecular biology research laboratories worldwide. Phages make attractive new targets for the development of novel antimicrobials. While the number of phage genome depositions has increased in recent years, the expected bacteriophage diversity remains underrepresented. Here we demonstrate how undergraduates can contribute to the identification of novel phages and that a single City in England can provide ample phage diversity and the opportunity to find novel technologies. Moreover, we demonstrate that the interactions and intricacies of the interplay between bacterial phage defense systems such as Bacteriophage Exclusion (BREX) and phages are more complex than originally thought. Further work will be required in the field before the dynamic interactions between phages and bacterial defense systems are fully understood and integrated with novel phage therapies.

Dataset Information

Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater.

Background

Materials and methods

Results

Conclusions

Publications

Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater.

Similar Datasets

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