Co-expression of Foxp3 and Helios facilitates the identification of human T regulatory cells in health and disease.

ABSTRACT: Foxp3 is regarded as the major transcription factor for T regulatory (T_reg) cells and expression of Foxp3 is used to identify and quantitate Treg cells in mouse models. However, several studies have demonstrated that human CD4⁺ T conventional (T_conv) cells activated in vitro by T cell receptor (TCR) stimulation can express Foxp3. This observation has raised doubt as to the suitability of Foxp3 as a T_reg marker in man. Helios, a member of the Ikaros gene family, has been shown to be expressed by 80-90% of human Foxp3⁺ T_reg cells and can potentially serve as a marker of human T_reg. Here, we confirm that Foxp3 expression is readily upregulated by T_conv upon TCR stimulation in vitro, while Hel

SUBMITTER: Morina L 

PROVIDER: S-EPMC10282999 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature