Choice of buffer in mobile phase can substantially alter peak areas in quantification of lipids by HPLC-ELSD.

ABSTRACT: Evaporative light scattering detectors (ELSD) are commonly used with high-performance liquid chromatography (HPLC) to separate and quantify lipids, which are typically not easily detectable by more conventional methods such as UV-visible detectors. In many HPLC-ELSD methods to analyze lipids, a volatile buffer is included in the mobile phase to control the pH and facilitate separation between lipid species. Here, we report an unintended effect that buffer choice can have in HPLC-ELSD analysis of lipids - the identity and concentration of the buffer can substantially influence the resulting ELSD peak areas. To isolate this effect, we use a simple isocratic methanol mobile phase supplemented with different concentrations of commonly used buffers for ELSD analysis, and quantify the effect on

SUBMITTER: Graceffa O 

PROVIDER: S-EPMC10283026 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature