A comparative study of gastric adenocarcinoma HER2 IHC phenotype and mass spectrometry-based quantification.

ABSTRACT:

Introduction

Gastric cancer is a highly heterogeneous malignant tumor of the digestive system. Anti-HER2 treatment can inhibit downstream signaling pathways and improve clinical treatment and outcomes in patients with HER2 protein overexpression. Currently, two standard methods for evaluating HER2 expression status are immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). However, these low-throughput assays often produce discordant or equivocal results.

Methods

In this study, we presented a new HER2 protein detection method based on mass spectrometry selected reaction monitoring (MS-SRM) and validated the method. We conducted a retrospective study on 118 formalin-fixed paraffin-embedded (FFPE) tissues from patients with advanced gastric adenocarcinoma in

SUBMITTER: Xu B 

PROVIDER: S-EPMC10283037 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature