Dataset Information
MASH Native: a unified solution for native top-down proteomics data processing.
Ontology highlight
ABSTRACT: Motivation
Native top-down proteomics (nTDP) integrates native mass spectrometry (nMS) with top-down proteomics (TDP) to provide comprehensive analysis of protein complexes together with proteoform identification and characterization. Despite significant advances in nMS and TDP software developments, a unified and user-friendly software package for analysis of nTDP data remains lacking.Results
We have developed MASH Native to provide a unified solution for nTDP to process complex datasets with database searching capabilities in a user-friendly interface. MASH Native supports various data formats and incorporates multiple options for deconvolution, database searching, and spectral summing to provide a "one-stop shop" for characterizing both native protein complexes and proteoforms.Availability and implementation
The MASH Native app, video tutorials, written tutorials, and additional documentation are freely available for download at https://labs.wisc.edu/gelab/MASH_Explorer/MASHSoftware.php. All data files shown in user tutorials are included with the MASH Native software in the download .zip file.
SUBMITTER: Larson EJ
PROVIDER: S-EPMC10283151 | biostudies-literature | 2023 Jun
REPOSITORIES: biostudies-literature
Publications
MASH Native: a unified solution for native top-down proteomics data processing.
Bioinformatics (Oxford, England) 20230601 6
<h4>Motivation</h4>Native top-down proteomics (nTDP) integrates native mass spectrometry (nMS) with top-down proteomics (TDP) to provide comprehensive analysis of protein complexes together with proteoform identification and characterization. Despite significant advances in nMS and TDP software developments, a unified and user-friendly software package for analysis of nTDP data remains lacking.<h4>Results</h4>We have developed MASH Native to provide a unified solution for nTDP to process complex ...[more]