Dynamic frustrated charge hotspots created by charge density modulation sequester globular proteins into complex coacervates.

ABSTRACT: This study presents a simple strategy for the sequestration of globular proteins as clients into synthetic polypeptide-based complex coacervates as a scaffold, thereby recapitulating the scaffold-client interaction found in biological condensates. Considering the low net charges of scaffold proteins participating in biological condensates, the linear charge density (σ) on the polyanion, polyethylene glycol-b-poly(aspartic acids), was reduced by introducing hydroxypropyl or butyl moieties as a charge-neutral pendant group. Complex coacervate prepared from the series of reduced-σ polyanions and the polycation, homo-poly-l-lysine, could act as a scaffold that sequestered various globular proteins with high encapsulation efficiency (>80%), which sometimes involved further

SUBMITTER: K C B 

PROVIDER: S-EPMC10283495 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature