Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array.

ABSTRACT:

Significance

Fluorescence lifetime imaging microscopy (FLIM) of the metabolic co-enzyme nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] is a popular method to monitor single-cell metabolism within unperturbed, living 3D systems. However, FLIM of NAD(P)H has not been performed in a light-sheet geometry, which is advantageous for rapid imaging of cells within live 3D samples.

Aim

We aim to design, validate, and demonstrate a proof-of-concept light-sheet system for NAD(P)H FLIM.

Approach

A single-photon avalanche diode camera was integrated into a light-sheet microscope to achieve optical sectioning and limit out-of-focus contributions for NAD(P)H FLIM of single cells.

Results

An NAD(P)H light-sheet FLIM system was built and validated with fluorescence lif

SUBMITTER: Samimi K 

PROVIDER: S-EPMC10284079 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature