De novo glycan sequencing by electronic excitation dissociation MS²-guided MS³ analysis on an Omnitrap-Orbitrap hybrid instrument.

ABSTRACT: Comprehensive de novo glycan sequencing remains an elusive goal due to the structural diversity and complexity of glycans. Present strategies employing collision-induced dissociation (CID) and higher energy collisional dissociation (HCD)-based multi-stage tandem mass spectrometry (MSⁿ) or MS/MS combined with sequential exoglycosidase digestions are inherently low-throughput and difficult to automate. Compared to CID and HCD, electron transfer dissociation (ETD) and electron capture dissociation (ECD) each generate more cross-ring cleavages informative about linkage positions, but electronic excitation dissociation (EED) exceeds the information content of all other methods and is also applicable to analysis of singly charged precursors. Although EED can provide exte

SUBMITTER: Wei J 

PROVIDER: S-EPMC10284134 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature