Scalable cryopreservation of infectious Cryptosporidium hominis oocysts by vitrification.

ABSTRACT: Cryptosporidium hominis is a serious cause of childhood diarrhea in developing countries. The development of therapeutics is impeded by major technical roadblocks including lack of cryopreservation and simple culturing methods. This impacts the availability of optimized/standardized singular sources of infectious parasite oocysts for research and human challenge studies. The human C. hominis TU502 isolate is currently propagated in gnotobiotic piglets in only one laboratory, which limits access to oocysts. Streamlined cryopreservation could enable creation of a biobank to serve as an oocyst source for research and distribution to other investigators requiring C. hominis. Here, we report cryopreservation of C. hominis TU502 oocysts by vitrification using specially designed specimen containe

SUBMITTER: Jaskiewicz JJ 

PROVIDER: S-EPMC10284403 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature