Munc13- and SNAP25-dependent molecular bridges play a key role in synaptic vesicle priming.

ABSTRACT: Synaptic vesicle tethering, priming, and neurotransmitter release require a coordinated action of multiple protein complexes. While physiological experiments, interaction data, and structural studies of purified systems were essential for our understanding of the function of the individual complexes involved, they cannot resolve how the actions of individual complexes integrate. We used cryo-electron tomography to simultaneously image multiple presynaptic protein complexes and lipids at molecular resolution in their native composition, conformation, and environment. Our detailed morphological characterization suggests that sequential synaptic vesicle states precede neurotransmitter release, where Munc13-comprising bridges localize vesicles <10 nanometers and soluble N-ethylmaleimide

SUBMITTER: Papantoniou C 

PROVIDER: S-EPMC10284560 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature