Pasteurization of human milk affects the miRNA cargo of EVs decreasing its immunomodulatory activity.

ABSTRACT: In this report, we evaluated the effect of the pasteurization (P) process of mother's own milk (MOM) on the miRNA content of extracellular vesicles (EVs) and its impact on innate immune responses. Differences in size or particle number were not observed upon pasteurization of MOM (PMOM). However, significant differences were observed in the EV membrane marker CD63 and miRNA profiles. miRNA sequencing identified 33 differentially enriched miRNAs between MOM_EV and PMOM_EV. These changes correlated with significant decreases in the ability of PMOM_EV to modulate IL-8 secretion in intestinal Caco2 cells where only MOM_EV were able to decrease IL-8 secretion in presence of TNFα. While EVs from MOM_EV and PMOM_EV were both able to indu

SUBMITTER: Torrez Lamberti MF 

PROVIDER: S-EPMC10284810 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature