Reprogramming the metabolism of an acetogenic bacterium to homoformatogenesis.

ABSTRACT: Methyl groups are abundant in anoxic environments and their utilization as carbon and energy sources by microorganisms involves oxidation of the methyl groups to CO₂, followed by transfer of the electrons to an acceptor. In acetogenic bacteria, the electron acceptor is CO₂ that is reduced to enzyme bound carbon monoxide, the precursor of the carboxyl group in acetate. Here, we describe the generation of a mutant of the acetogen Acetobacterium woodii in which the last step in methyl group oxidation, formate oxidation to CO₂ catalyzed by the HDCR enzyme, has been genetically deleted. The mutant grew on glycine betaine as methyl group donor, and in contrast to the wild type, formed formate alongside acetate, in a 1:2 ratio, demonstrating that methyl group oxid

SUBMITTER: Moon J 

PROVIDER: S-EPMC10284823 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature