Enhancement of the activity of the antimicrobial peptides HNP1 and LL-37 by bovine pancreatic ribonuclease A.

ABSTRACT: Background: HNP1, LL-37, and HBD1 are antimicrobial against Escherichia coli ATCC 25922 at the standard inoculum but less active at higher inocula.   Methods: The virtual colony count (VCC) microbiological assay was adapted for high inocula and the addition of yeast tRNA and bovine pancreatic ribonuclease A (RNase).  96-well plates were read for 12 hours in a Tecan Infinite M1000 plate reader and photographed under 10x magnification.    Results: Adding tRNA 1:1 wt/wt to HNP1 at the standard inoculum almost completely abrogated activity.  Adding RNase 1:1 to HNP1 at the standard inoculum of 5x10 ⁵ CFU/mL did not enhance activity.  Increasing the inoculum to 6.25x10 ⁷ CFU/mL almost abrogated HNP1 activity.  However, adding RNase 25:1 to HNP1 enhance

SUBMITTER: Ericksen B 

PROVIDER: S-EPMC10285323 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature