Mast cells contribute to the resolution of allergic inflammation by releasing resolvin D1.

ABSTRACT:

Background

Mast cells are initiators and main effectors of allergic inflammation, together with eosinophils, with whom they can interact in a physical and soluble cross-talk with marked pro-inflammatory features, the Allergic Effector Unit. The pro-resolution role of mast cells, alone or in co-culture with eosinophils, has not been characterized yet.

Objectives

We aimed to investigate select pro-resolution pathways in mast cells in vitro and in vivo in allergic inflammation.

Methods

In vitro, we employed human and murine mast cells and analyzed release of resolvin D1 and expression of 15-lipoxygenase after IgE-mediated activation. We performed co-culture of IgE-activated mast cells with peripheral blood eosinophils and investigated 15-lipoxygenase expression and Resol

SUBMITTER: Puzzovio PG 

PROVIDER: S-EPMC10285510 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature