Characterization of an air-liquid interface primary human vaginal epithelium to study Ebola virus infection and testing of antivirals.

ABSTRACT: Ebola virus (EBOV) is the causative agent of the often-fatal Ebola virus disease (EVD) characterized by hemorrhagic fever in humans and non-human primates. Sexual transmission from male survivors has been at the origin of multiple outbreak flare-ups between 2015 and 2021. However, this route is still poorly understood and the resulting EVD from it is also understudied. To support epidemiological studies documenting sexual transmission to women, and as a transition from previously using monolayer vaginal epithelial cells (VK2/E6E7), we first determined the biological relevance of two similar air-liquid interface models of the human vaginal epithelium (VEC and VLC Epivaginal™) and then characterized their susceptibility to EBOV and virus-induced inflammation. Finally, we evaluated toxicity o

SUBMITTER: Escaffre O 

PROVIDER: S-EPMC10286122 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature