RNA-Seq-based discovery of genetic variants and allele-specific expression of two layer lines and broiler chicken.

ABSTRACT: Recent advances in the selective breeding of broilers and layers have made poultry production one of the fastest-growing industries. In this study, a transcriptome variant calling approach from RNA-seq data was used to determine population diversity between broilers and layers. In total, 200 individuals were analyzed from three different chicken populations (Lohmann Brown (LB), n = 90), Lohmann Selected Leghorn (LSL, n = 89), and Broiler (BR, n = 21). The raw RNA-sequencing reads were pre-processed, quality control checked, mapped to the reference genome, and made compatible with Genome Analysis ToolKit for variant detection. Subsequently, pairwise fixation index (F _ST) analysis was performed between broilers and layers. Numerous candidate genes were

SUBMITTER: Iqbal MA 

PROVIDER: S-EPMC10286233 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature