Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China.

ABSTRACT:

Objective

To isolate a prevalent G9P[8] group A rotavirus (RVA) (N4006) in China and investigate its genomic and evolutionary characteristics, with the goal of facilitating the development of a new rotavirus vaccine.

Methods

The RVA G9P[8] genotype from a diarrhea sample was passaged in MA104 cells. The virus was evaluated by TEM, polyacrylamide gel electrophoresis, and indirect immunofluorescence assay. The complete genome of virus was obtained by RT-PCR and sequencing. The genomic and evolutionary characteristics of the virus were evaluated by nucleic acid sequence analysis with MEGA ver. 5.0.5 and DNASTAR software. The neutralizing epitopes of VP7 and VP4 (VP5* and VP8*) were analyzed using BioEdit ver. 7.0.9.0 and PyMOL ver. 2.5.2.

Results

The RVA N4006 (G9P[8] ge

SUBMITTER: Peng R 

PROVIDER: S-EPMC10286334 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature