Immunohistochemical detection of MUC5AC and MUC5B mucins in ferrets.

ABSTRACT:

Objective

Cystic fibrosis (CF) is a genetic condition that causes abnormal mucus secretions in affected organs. MUC5AC and MUC5B are gel-forming mucins and frequent targets for investigations in CF tissues. Our objective was to qualify MUC5AC and MUC5B immunohistochemical techniques to provide a useful tool to identify, localize and interpret mucin expression in ferret tissues.

Results

MUC5AC and MUC5B mucins were detected most commonly in large airways and least in small airways, consistent with reported goblet cell density in airway surface epithelia. We evaluated whether staining method affected the detection of goblet cell mucins in serial sections of bronchial surface epithelia. Significant differences between stains were not observed suggesting common co-expression MUC

SUBMITTER: Meyerholz DK 

PROVIDER: S-EPMC10286488 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature