N⁶-methyladenosine is required for efficient RNA synthesis of Ebola virus and other haemorrhagic fever viruses.

ABSTRACT: N⁶-methyladenosine (m⁶A) is one of the most abundant modifications of cellular RNA, where it serves various functions. m⁶A methylation of many viral RNA species has also been described; however, little is known about the m⁶A epitranscriptome of haemorrhagic fever-causing viruses like Ebola virus (EBOV). Here, we analysed the importance of the methyltransferase METTL3 for the life cycle of this virus. We found that METTL3 interacts with the EBOV nucleoprotein and the transcriptional activator VP30 to support viral RNA synthesis, and that METTL3 is recruited into EBOV inclusions bodies, where viral RNA synthesis occurs. Analysis of the m⁶A methylation pattern of EBOV mRNAs showed that they are methylated by METTL3. Further studies reveal

SUBMITTER: Wendt L 

PROVIDER: S-EPMC10286672 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature