Negatively Competitive Incoherent Feedforward Loops Mitigate Winner-Take-All Resource Competition.

ABSTRACT: The effects of host resource limitations on the function of synthetic gene circuits have gained significant attention over the past years. Hosts, having evolved resource capacities optimal for their own genome, have been repeatedly demonstrated to suffer from the added burden of synthetic genetic programs, which may in return pose deleterious effects on the circuit's function. Three resource controller archetypes have been proposed previously to mitigate resource distribution problems in dynamic circuits: the local controller, the global controller, and a "negatively competitive" regulatory (NCR) controller that utilizes synthetic competition to combat resource competition. The dynamics of negative feedback forms of these controllers have been previously investigated, and here we extend th

SUBMITTER: Stone A 

PROVIDER: S-EPMC10286714 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature