Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls.

ABSTRACT: Blood extracellular vesicles (EVs) play essential roles in cell-cell communication and their molecular cargo is a promising source of disease biomarkers. However, proteomic characterization of plasma-derived EVs is challenged by the presence of highly abundant plasma proteins, which limits the detection of less abundant proteins, and by the low number of EVs in biological fluids. The aim of this study was to investigate if the removal of abundant plasma proteins prior to EV isolation could improve plasma-derived EV characterization by LC-MS/MS and expand the proteome coverage. Plasma depletion was performed using a single-use spin column and EVs were isolated from only 100 µL of non-depleted and depleted plasma by size exclusion chromatography. Afterwards, EVs were characterized by nanopar

SUBMITTER: Reymond S 

PROVIDER: S-EPMC10287573 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature