Cross-reactivity trends when selecting scFv antibodies against snake toxins using a phage display-based cross-panning strategy.

ABSTRACT: Antibodies with cross-reactive binding and broad toxin-neutralizing capabilities are advantageous for treating indications such as infectious diseases and animal envenomings. Such antibodies have been successfully selected against closely related antigens using phage display technology. However, the mechanisms driving antibody cross-reactivity typically remain to be elucidated. Therefore, we sought to explore how a previously reported phage display-based cross-panning strategy drives the selection of cross-reactive antibodies using seven different snake toxins belonging to three protein (sub-)families: phospholipases A₂, long-chain α-neurotoxins, and short-chain α-neurotoxins. We showcase how cross-panning can increase the chances of discovering cross-reactive single-chain varia

SUBMITTER: Sorensen CV 

PROVIDER: S-EPMC10287648 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature