Quantification methods of determining brewer's and pharmaceutical yeast cell viability: accuracy and impact of nanoparticles.

ABSTRACT: For industrial processes, a fast, precise, and reliable method of determining the physiological state of yeast cells, especially viability, is essential. However, an increasing number of processes use magnetic nanoparticles (MNPs) for yeast cell manipulation, but their impact on yeast cell viability and the assay itself is unclear. This study tested the viability of Saccharomyces pastorianus ssp. carlsbergensis and Pichia pastoris by comparing traditional colourimetric, high-throughput, and growth assays with membrane fluidity. Results showed that methylene blue staining is only reliable for S. pastorianus cells with good viability, being erroneous in low viability (R² = 0.945; [Formula: see text] = 5.78%). In comparison, the fluorescence microscopy-based assay of S. pastorianus

SUBMITTER: Eigenfeld M 

PROVIDER: S-EPMC10287788 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature