Estimation of measurement uncertainty for the quantification of protein by ID-LC-MS/MS.

ABSTRACT: The emergence of mass spectrometry (MS)-based methods to quantify proteins for clinical applications has led to the need for accurate and consistent measurements. To meet the clinical needs of MS-based protein results, it is important that the results are traceable to higher-order standards and methods and have defined uncertainty values. Therefore, we outline a comprehensive approach for the estimation of measurement uncertainty of a MS-based procedure for the quantification of a protein biomarker. Using a bottom-up approach, which is the model outlined in the "Guide to the Expression of Uncertainty of Measurement" (GUM), we evaluated the uncertainty components of a MS-based measurement procedure for a protein biomarker in a complex matrix. The cause-and-effect diagram of the procedure is

SUBMITTER: Beasley-Green A 

PROVIDER: S-EPMC10287794 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature