Age-associated polyamines in peripheral blood cells and plasma in 20 to 70 years of age subjects.

ABSTRACT: Dietary polyamines have been associated with slowing ageing processes and various pathologies, raising the importance of establishing reference values at different ages throughout life. This study aimed to analyse age-dependent variations in polyamine content using peripheral blood cells and plasma in a healthy and homogeneous population. Peripheral blood of 193 volunteers of both sexes (20-70 years), selected by convenience, was processed to separate cells and plasma. A pre-column derivatization method was used to determine the amines by HPLC (nmol or pmol/mg protein or nmol/ml) to analyse their association with the age (continuous or ordinal in decades) of the subjects. Putrescine and spermine weakly declined significantly in mononuclear cells with age. In erythrocytes and plasma, putres

SUBMITTER: Sanchez M 

PROVIDER: S-EPMC10287822 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature