DNA binding specificity of all four Saccharomyces cerevisiae forkhead transcription factors.

ABSTRACT: Quantifying the nucleotide preferences of DNA binding proteins is essential to understanding how transcription factors (TFs) interact with their targets in the genome. High-throughput in vitro binding assays have been used to identify the inherent DNA binding preferences of TFs in a controlled environment isolated from confounding factors such as genome accessibility, DNA methylation, and TF binding cooperativity. Unfortunately, many of the most common approaches for measuring binding preferences are not sensitive enough for the study of moderate-to-low affinity binding sites, and are unable to detect small-scale differences between closely related homologs. The Forkhead box (FOX) family of TFs is known to play a crucial role in regulating a variety of key processes from proliferation and

SUBMITTER: Cooper BH 

PROVIDER: S-EPMC10287902 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature