Aquaporin 9 is involved in CRC metastasis through DVL2-dependent Wnt/β-catenin signaling activation.

ABSTRACT:

Background

Aquaporin 9 (AQP9) is permeable to water or other small molecules, and plays an important role in various cancers. We previously found that AQP9 was related to the efficacy of chemotherapy in patients with colorectal cancer (CRC). This study aimed to identify the role and regulatory mechanism of AQP9 in CRC metastasis.

Methods

The clinical significance of AQP9 was analysed by using bioinformatics and tissue microarray. Transcriptome sequencing, Dual-Luciferase Reporter Assay, Biacore, and co-immunoprecipitation were employed to demonstrate the regulatory mechanism of AQP9 in CRC. The relationship between AQP9 and CRC metastasis was verified in vitro and in vivo by using real-time cell analysis assay, high content screening, and liver metastasis model

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10287913 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature