AT-specific DNA visualization revisits the directionality of bacteriophage λ DNA ejection.

ABSTRACT: In this study, we specifically visualized DNA molecules at their AT base pairs after in vitro phage ejection. Our AT-specific visualization revealed that either end of the DNA molecule could be ejected first with a nearly 50% probability. This observation challenges the generally accepted theory of Last In First Out (LIFO), which states that the end of the phage λ DNA that enters the capsid last during phage packaging is the first to be ejected, and that both ends of the DNA are unable to move within the extremely condensed phage capsid. To support our observations, we conducted computer simulations that revealed that both ends of the DNA molecule are randomized, resulting in the observed near 50% probability. Additionally, we found that the length of the ejected DNA by LIFO was consistent

SUBMITTER: Bong S 

PROVIDER: S-EPMC10287942 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature