Dynamic accumulation of cyclobutane pyrimidine dimers and its response to changes in DNA conformation.

ABSTRACT: Photochemical dimerization of adjacent pyrimidines is fundamental to the creation of mutagenic hotspots caused by ultraviolet light. Distribution of the resulting lesions (cyclobutane pyrimidine dimers, CPDs) is already known to be highly variable in cells, and in vitro models have implicated DNA conformation as a major basis for this observation. Past efforts have primarily focused on mechanisms that influence CPD formation and have rarely considered contributions of CPD reversion. However, reversion is competitive under the standard conditions of 254 nm irradiation as illustrated in this report based on the dynamic response of CPDs to changes in DNA conformation. A periodic profile of CPDs was recreated in DNA held in a bent conformation by λ repressor. After linearization of this DNA, t

SUBMITTER: Moirangthem R 

PROVIDER: S-EPMC10287945 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature