Millimetric scale two-photon Bessel-Gauss beam light sheet microscopy with three-axis isotropic resolution using an axicon lens.

ABSTRACT:

Significance

Typical light sheet microscopes suffer from artifacts related to the geometry of the light sheet. One main inconvenience is the non-uniform thickness of the light sheet obtained with a Gaussian laser beam.

Aim

We developed a two-photon light sheet microscope that takes advantage of a thin and long Bessel-Gauss beam illumination to increase the sheet extent without compromising the resolution.

Approach

We use an axicon lens placed directly at the output of an amplified femtosecond laser to produce a long Bessel-Gauss beam on the sample. We studied the dopaminergic system and its projections in a whole cleared mouse brain.

Results

Our light sheet microscope allows an isotropic resolution of 2.4  μm in all three axes of the scanned volume while keepin

SUBMITTER: Akitegetse C 

PROVIDER: S-EPMC10288127 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature