Design and Evolution of Enhanced Peptide-Peptide Ligation for Modular Transglutaminase Assembly.

ABSTRACT: Robust and precise tools are needed to enhance the functionality and resilience of synthetic nanoarchitectures. Here, we have employed directed evolution and rational design to build a fast-acting molecular superglue from a bacterial adhesion protein. We have generated the SnoopLigase2 coupling system, a genetically encoded route for efficient transamidation between SnoopTag2 and DogTag2 peptides. Each peptide was selected for rapid reaction by phage display screening. The optimized set allows more than 99% completion and is compatible with diverse buffers, pH values, and temperatures, accelerating the reaction over 1000-fold. SnoopLigase2 directs a specific reaction in the mammalian secretory pathway, allowing covalent display on the plasma membrane. Transglutaminase 2 (TG2) has a network

SUBMITTER: Keeble AH 

PROVIDER: S-EPMC10288437 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature